Muaj ntau cov cell tawg tom qab mechanical lysis ntawm cell suspension.Yuav ua li cas tshem tawm cov tawg no?Cia wb mus saib ntawm ntau txoj hauv kev:

1. Siv txoj kev dilution.Thaum cov hlwb tau diluted, lawv tuaj yeem loj hlob tuaj, lawv yuav dhau los ua ntau thiab ntau dua, thiab cov khib nyiab ntawm tes yuav ua rau tsawg dua.
2. Tseem muaj kev sib haum xeeb.Cells nyob sai dua feem ntau fragments: : Tsiv lub cell ncua mus rau hauv lubcentrifuge tub, thiab thaum feem ntau ntawm cov hlwb dab dej, cov tshuaj sab saud tuaj yeem nqus tau, thiab tom qab ntawd ntxiv rau cov kab lis kev cai daws kom tshem tawm cov hlwb.Txoj kev no tuaj yeem siv tau ntau zaus, thaum txhua lub sijhawm koj tuaj yeem soj ntsuam seb nws puas ua tau raws li koj qhov yuav tsum tau ua hauv lub tshuab tsom.
3. Tsawg-ceev centrifugetuaj yeem tshem tawm cov khib nyiab, feem ntau 700g, 5 mins
4. Thaum centrifuging, nyob rau hauv cov xwm txheej uas muaj pes tsawg leeg txaus, txo lub sij hawm centrifugation, xws li 5min, 1000rpm, es tsis txhob 3min, 1000rpm, thiab tshem tawm cov supernatant, vim necrosis thiab khib nyiab feem ntau nyob rau hauv lub supernatant!Qhov kev ua haujlwm no tsuas yog yuav tsum tau ua ib zaug thaum lub sijhawm ntxuav ua ntej incubation!

Txawm hais tias muaj ntau txoj hauv kev los tshem tawm cov khib nyiab ntawm tes, koj yuav pom tias centrifugation yog ib txoj hauv kev zoo tshaj plaws!

Thiab vim tias cov ntaub so ntswg thiab cov hlwb muaj cov phab ntsa ntawm tes uas muaj cellulose, hemicellulose thiab pectin, feem ntau yuav tsum tau zom quartz xuab zeb lossis iav hmoov nrog cov tshuaj tsim nyog los yog kho nrog cellulase kom ua tiav lub hom phiaj.Bacterial cell fragmentation yog qhov nyuaj dua, vim hais tias tag nrho cov kab mob ntawm cov phab ntsa pob txha pob txha yog covalent daim ntawv cog lus ntawm peptidoglycan cystic macromolecules, tawv heev.Cov txheej txheem ntawm kev rhuav cov kab mob ntawm cov phab ntsa muaj xws li ultrasonic crushing, xuab zeb sib tsoo, siab extrusion los yog lysozyme kho.Tom qab cov ntaub so ntswg thiab cov hlwb tawg, qhov tsim nyog tsis raug xaiv los rho tawm cov protein uas xav tau.Insoluble cov ntaub ntawv xws li cell fragments raug tshem tawm los ntawmcentrifugelos yog lim.

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