• lab-217043_1280

Ua rau kev tsom xam ntawm precipitate hauv cell culture flask-temperature

Cell kab lis kev cai yog ib txoj hauv kev rau cov hlwb kom muaj sia nyob, loj hlob, tsim thiab tswj lawv cov qauv tseem ceeb thiab kev ua haujlwm los ntawm kev ua raws li ib puag ncig hauv vivo hauv vitro.Cell kab lis kev cai lub raj misyog ib hom cell consumable feem ntau siv nyob rau hauv adherent cell kab lis kev cai.Nyob rau hauv tus txheej txheem ntawm cell kab lis kev cai, peb feem ntau pom ib co impurities tsub zuj zuj nyob rau hauv cov kua.Muaj ntau qhov laj thawj rau qhov xwm txheej no, thiab qhov kub thiab txias kuj yog ib qho laj thawj.
95Lub xub ntiag ntawm nag lossis daus nyob rau hauv lub cell culture flask tej zaum yuav tshwm sim ntawm cell kab mob.Yog tias tsis suav nrog kev sib kis, turbidity hauv cell kab lis kev cai nruab nrab feem ntau txhais tau tias nag lossis daus ntawm cov hlau, cov protein, thiab lwm yam nruab nrab.Feem ntau precipitates impair ib txwm cell proliferation vim lawv hloov cov muaj pes tsawg leeg ntawm nruab nrab los ntawm chelating as-ham thiab lwm yam uas yuav tsum tau.Cov nag lossis daus tuaj yeem pom microscopically thiab tuaj yeem cuam tshuam nrog cov kev sim uas xav tau kev tshuaj ntsuam xyuas.
 
Hauv kab lis kev cai ntawm tes, qhov kub thiab txias yog ib qho tseem ceeb ua rau nag lossis daus.Thaum qhov kub thiab txias hloov siab heev, siab molecular hnyav plasma proteins yuav precipitated los ntawm cov tshuaj.Thaum tshav kub kub inactivation thiab khov-thaw lub voj voog tuaj yeem txhawb nqa protein degradation thiab nag lossis daus.Vim hais tias cov kua los yog qhov nruab nrab rov tsim dua yog khaws cia hauv qhov txias txias ntawm kev siv, ntsev yuav daws tau, tshwj xeeb tshaj yog nyob rau hauv 10X los yog lwm yam kev daws teeb meem.
 
Tau kawg, nag lossis daus tshwm hauv lub raj mis kab lis kev cai ntawm tes.Yog tias nws tau txiav txim siab tias qhov kub thiab txias yog qhov ua rau, kev saib xyuas yuav tsum tau them rau qhov chaw cia khoom thiab kev ua haujlwm ntawm cov kab lis kev cai nruab nrab kom tsis txhob rov khov thiab thawing, uas tuaj yeem txo qhov tshwm sim ntawm nag lossis daus.


Post lub sij hawm: Sep-06-2022