Cell Hoobkas yog ib qho khoom siv hauv cov kab lis kev cai loj ntawm tes, uas yog siv rau cov kab lis kev cai ntawm tes.Cell loj hlob xav tau txhua yam ntawm cov as-ham, yog li lawv yog dab tsi?
1. Kab lis kev cai nruab nrab
Lub cell kab lis kev cai nruab nrab muab cov cell nyob rau hauv lub cell Hoobkas nrog cov as-ham uas yuav tsum tau rau kev loj hlob, nrog rau cov carbohydrates, amino acids, inorganic ntsev, vitamins, thiab lwm yam. Muaj ntau yam khoom hluavtaws muaj rau cov khoom noj khoom haus ntawm cov hlwb sib txawv, xws li EBSS. , Eagle, MEM, RPMll640, DMEM, thiab lwm yam.

2. Lwm cov khoom xyaw ntxiv
Ntxiv nrog rau cov khoom noj khoom haus yooj yim muab los ntawm ntau yam khoom siv hluav taws xob, lwm yam khoom siv, xws li cov ntshav qab zib thiab cov khoom, yuav tsum tau ntxiv raws li cov hlwb sib txawv thiab cov hom phiaj sib txawv.
Serum muab cov khoom tseem ceeb xws li extracellular matrix, kev loj hlob yam thiab transferrin, thiab fetal bovine serum feem ntau yog siv.Qhov kev faib ua feem ntawm cov ntshav yuav tsum tau ntxiv yog nyob ntawm lub cell thiab lub hom phiaj ntawm kev kawm.10% ~ 20% serum tuaj yeem tswj kev loj hlob sai thiab loj hlob ntawm cov hlwb, hu ua qhov nruab nrab loj hlob;Txhawm rau kom muaj kev loj hlob qeeb lossis tsis txawj tuag ntawm cov hlwb, 2% ~ 5% cov ntshav tuaj yeem ntxiv, hu ua kev saib xyuas kab lis kev cai.
Glutamine yog ib qho tseem ceeb nitrogen rau kev loj hlob ntawm tes thiab ua lub luag haujlwm tseem ceeb hauv cov txheej txheem ntawm kev loj hlob ntawm tes thiab cov metabolism.Txawm li cas los xij, vim tias glutamine tsis ruaj khov thiab yooj yim rau degrade hauv cov tshuaj, nws tuaj yeem decompose txog 50% tom qab 7 hnub ntawm 4 ℃, yog li glutamine yuav tsum tau ntxiv ua ntej siv.
Feem ntau hais lus, ntau yam xov xwm thiab cov ntshav qab zib tau siv rau hauv kab lis kev cai ntawm tes, tab sis txhawm rau tiv thaiv kev kis kab mob ntawm tes thaum kab lis kev cai, qee yam tshuaj tua kab mob, xws li penicillin, streptomycin, gentamicin, thiab lwm yam, kuj tau ntxiv rau hauv xov xwm.