Liquid strain yog hom kab lis kev cai nrog cov kab lis kev cai nruab nrab.Nws muaj qhov zoo ntawm lub voj voog luv luv, cov kab mob muaj hnub nyoog zoo ib yam, yooj yim inoculation, haum rau lub Hoobkas tsim, thiab lwm yam.Nws tau txais tos los ntawm feem ntau ntawm cov neeg cog qoob loo.Cell shaker (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/) yog ib yam khoom siv uas yuav tsum tau muaj nyob rau hauv kab lis kev cai ntawm cov kua hom.Cov ntsiab lus hauv qab no yuav tsum tau ua tib zoo saib rau hauv kab lis kev cai:

1.Kev npaj ntawm kab lis kev cai nruab nrab raws li cov qauv ntawm ntau yam khoom, rau hauv lub thawv, yaj nrog dej, muab faib ua peb lub raj mis, feem ntau 250 ~ 300mL cell co lub raj mis 50mL kab lis kev cai nruab nrab,500ml daim duab peb sab lub raj mis  (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/)100mL kab lis kev cai nruab nrab, ntsaws paj rwb ntsaws, nrog ntawv xov xwm qhwv;Kuj tseem tuaj yeem ua los ntawm 8 txheej ntawm gauze 8cm × 8cm lub raj mis stopper sealing, sab nraum nrog cov ntawv xim av sealing.
2. Autoclave sterilization txoj kev feem ntau yog siv rau kev ua kom tsis muaj menyuam, uas yuav tsum tau 121 ℃ thiab 30min.Tshem tawm thiab txias kom txog 30 ℃.Muab tso rau hauv chav tsis muaj menyuam lossis lub thawv inoculation rau kev siv.
3. Raws li kev ua haujlwm aseptic, txhua lub raj mis tuaj yeem txuas nrog 2 ~ 3cm2 slant strain, thiab txhua qhov slant strain tuaj yeem txuas nrog 4 ~ 5 fwj.Nws yog qhov zoo dua los nqa qee qhov nruab nrab nrog cov kab ntxiv, kom lawv tuaj yeem ntab rau saum npoo ntawm qhov nruab nrab.Tom qab inoculation, npog lub qhov ncauj ntawm lub raj mis nrog lub gauze thawj ntsaws, thiab khi nws ruaj khov nrog hlua.
4. Lub raj mis inoculated strain tuaj yeem muab tso rau hauv lub txaj txaj rau kab lis kev cai, lossis tuaj yeem muab tso rau hauv qhov kub thiab txias ntawm 24 ~ 26 ℃ rau 48h, thiab tom qab oscillated kab lis kev cai tuaj yeem nqa tawm tom qab aerogenic mycelium txuas mus rau hauv kab lis kev cai nruab nrab. .Lub oscillating zaus ntawm reciprocating shaker yog 80 ~ 120r / min, thiab lub teb shaker yog 150 ~ 220r / min.3 ~ 4d tuaj yeem ua rau hauv lub shaker.Thaum kawg ntawm kab lis kev cai, vim muaj ntau hom, kab lis kev cai nruab nrab tshwm sim xim sib txawv, xws li oyster nceb, enoki nceb kab lis kev cai nruab nrab yog lub teeb daj;Nceb, ericius erinaceus kab lis kev cai nruab nrab yog xim av xim av, thiab nceb tshuaj tsw qab;Cov kab mob fungus nruab nrab yog xim av, tuab thiab qab zib.Cov kab lis kev cai nruab nrab tuaj yeem siv rau kev tsim lossis txuas ntxiv kab lis kev cai tsuas yog thaum qhov hnyav ntawm mycelium ncav cuag 10g / L thiab txoj kab uas hla ntawm mycelium pellets yog 1 ~ 2 hli.
 
5. Secondary kua strain ntau lawm theem nrab kua strain kab lis kev cai nruab nrab zus tau tej cov tib theem ntawm cov kua hom, cov kab lis kev cai thawv yuav tsum loj, lub peev xwm tsis ntau tshaj 3500mL.Tom qab sterilization thiab txias, cov fermented thawj cov kua hom tau muab tso rau hauv5L cell shaker(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/)ntawm qhov ratio ntawm 5% -10% thiab kab lis kev cai ntawm ib tug shaker ntawm ib tug qeeb ceev.Tom qab ib lub sij hawm ntawm oscillating kab lis kev cai, cov kua strains nrog uniform tis ntawm microspheres thiab ntshiab fermentation kua yuav tau.
Hauv cov ntsiab lus, thaum siv cell shaker rau kab lis kev cai kua hom, ua haujlwm raws li cov txheej txheem saum toj no, thiab ua tib zoo saib xyuas qhov ntsuas kub, co zaus thiab lwm yam hauv cov txheej txheem los xyuas kom meej cov kab lis kev cai.
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