Thaum peb siv qee cov kab lis kev cai ntawm tes, peb ib txwm ntsib teeb meem ntawm cell passage.Niaj hnub no, yuav qhia luv luv rau koj yuav ua li cas siv high-efficiency co flasks rau ntawm tes.Thaum peb siv high-efficiency co flasks(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/) rau kev hla ntawm tes, muaj ob txoj hauv kev rau koj xaiv los ntawm, xws li sau cov hlwb los ntawm centrifugation thiab tom qab ntawv, lossis ncaj qha txoj kev.

Txoj kev centrifugal passage:

(1) Hloov cov cell hauv lubhigh-efficiency shake flask ua ke nrog cov kab lis kev cai nruab nrab mus rau lub raj centrifuge rau centrifugation.

(2) Tshem tawm cov supernatant, ntxiv cov kab lis kev cai tshiab rau lub centrifuge tub thiabpibtsim kom muaj kev ncua ntawm tes.

(3) Suav thiab inoculate nyob rau hauv cov kab lis kev cai tshiab flasks feem.

Yog tias txoj cai ncaj qha tau txais kev pom zoo, cia cov hlwb raug tshem tawm maj mam nyob rau hauv qab ntawm lub tshuab ua haujlwm siab, nqus tawm 1/2 ~ 2/3 ntawm cov supernatant, thiab tom qab ntawd pipette los tsim lub cell ncua ua ntej dhau mus.

Dab tsi peb yuav tsum tau them sai sai rau thaum lub sijhawm ua haujlwm yog tias trypsin yuav tsum tau sov ua ntej, thiab qhov kub yog li 37 ° C.Lub centrifugation ceev yuav tsum tsim nyog.Yog tias qhov ceev tsawg dhau, cov hlwb tsis tuaj yeem sib cais tau zoo.Yog hais tias lub centrifugation ceev yog siab dhau lawm thiab lub sij hawm ntev heev, lub hlwb yuav raug nyem, ua kom puas los yog txawm tuag.Cov hlwb yuav tsum tau saib xyuas tsis tu ncua, thiab yog tias pom muaj kab mob sib kis, nws yuav tsum tau ua raws sijhawm.