PETG kab lis kev cai nruab nrab lub raj misyog lub raj mis yas siv dav.Nws lub raj mis lub cev yog pob tshab heev, txais cov qauv tsim square, lub teeb yuag, thiab tsis yooj yim tawg.Nws yog lub thawv ntim khoom zoo.Peb cov kev siv feem ntau yog cov hauv qab no peb:

1. Serum: Serum muab cov cell nrog cov as-ham hauv paus, kev loj hlob, kev khi cov protein, thiab lwm yam, kom tsis txhob muaj kev puas tsuaj rau cov hlwb, thiab tiv thaiv cov hlwb hauv kab lis kev cai.Cov kua dej rau lub sijhawm ntev yuav tsum tau muab cia rau hauv qhov chaw qis qis ntawm -20 ° C txog -70 ° C.Yog tias khaws cia hauv lub tub yees 4 ° C, feem ntau tsis pub ntau tshaj 1 lub hlis.

2.Culture nruab nrab: Cov kab lis kev cai nruab nrab feem ntau muaj cov carbohydrates, nitrogenous tshuaj, inorganic ntsev, vitamins thiab dej, thiab lwm yam. Nws tsis yog tsuas yog cov khoom siv tseem ceeb rau kev muab cov khoom noj ntawm tes thiab txhawb kev loj hlob ntawm tes, tab sis kuj tseem nyob ib puag ncig rau kev loj hlob ntawm tes thiab kev loj hlob. .Qhov chaw cia ntawm qhov nruab nrab yog 2 ° C-8 ° C, tiv thaiv los ntawm lub teeb.

3. Ntau yam reagents: Ntxiv nrog rau kev khaws cia ntawm cov ntshav qab zib thiab kab lis kev cai nruab nrab, PETG lub raj mis nruab nrab kuj tuaj yeem siv los ua cov thawv ntim rau ntau yam tshuaj lom neeg, xws li buffers, dissociation reagents, tshuaj tua kab mob, cell cryopreservation solutions, staining solutions, Growth additives, thiab lwm yam. Ib txhia ntawm cov reagents yuav tsum tau muab cia rau ntawm -20 ° C, thaum lwm tus yog khaws cia nyob rau hauv chav tsev kub.Tsis muaj teeb meem ib puag ncig twg, lub raj mis nruab nrab tuaj yeem ua tau raws li lawv cov kev cia khoom.

Lub raj mis nruab nrab PETG feem ntau yog siv los tuav peb qhov kev daws teeb meem saum toj no.Txhawm rau ua kom yooj yim pom qhov pom ntawm qhov ntim ntawm cov tshuaj, muaj qhov ntsuas ntawm lub raj mis lub cev.Cov kev daws teeb meem saum toj no yog siv rau hauv kab lis kev cai ntawm tes, thiab kev saib xyuas yuav tsum tau them rau kev ua haujlwm aseptic thaum ntxiv lawv.