Coverlenmeyer khob khobyog lub thawv kab lis kev cai tshwj xeeb rau kev ncua ntawm tes kab lis kev cai, thiab tseem tuaj yeem siv los npaj, sib tov thiab khaws ntau yam xov xwm.Cov kab lis kev cai no siv tau thaum culturing Jurkat hlwb.

Jurkat cell kab yog muab los ntawm cov ntshav peripheral ntawm ib tug me nyuam tub muaj hnub nyoog 14 xyoo thiab yog ib qho kev ncua ntawm tes.Jurkat-derived cell kab tsis muaj qee cov noob twb muaj nyob rau hauv cov tsev txhab nyiaj kab lis kev cai.Immortalized tib neeg T lymphocyte kab feem ntau yog siv los kawm mob T cell leukemia, T cell signaling, thiab ntau yam chemokine receptors raug rau kis kab mob, tshwj xeeb tshaj yog HIV qhia.Nws muaj ntau yam kev siv hauv kev tshawb fawb lom, xws li daim ntawv thov ntawm Jurkat hlwb los kawm M1-RNA ntawm ribonuclease P, thiab kev kawm ntawm M1-RNA ntawm anti-MHC chav kawm II transcriptional activator (CITA) los inhibit Kev nthuav qhia ntawm MHC class II molecules ntawm lub xov tooj ntawm tes.

Thaum culturing Jurkat hlwb hauv erlenmeyer co flasks, RPMI1640 nruab nrab, 10% FBS yuav tsum tau;Qhov kub yog tswj ntawm 37 ° C, 5% carbon dioxide, PH nqi 7.2-7.4, aseptic qhov kub thiab txias kab lis kev cai.So thiab tshuaj tua kab mob nrog 75% cawv ua ntej hloov mus rau lub cell ultra-huv lub rooj ntev zaum, tshem tawm lub cell cryovial los ntawm cov kua nitrogen tank, tam sim ntawd muab tso rau hauv ib tug 37 ° C dej da dej thiab co lub cell cryotube sai sai thaw nws sai.Tom qab ntawd, tom qab centrifugation, pipetting thiab sib tov, thiab lwm yam, nws tau muab tso rau hauv lub cell incubator rau cultivation.

Cells yog tshwj xeeb yog rhiab heev rau ib puag ncig.Thaum culturing Jurkat cells nyob rau hauv erlenmeyer cell co flasks, kev tu tus kheej yuav tsum ua kom zoo, yuav tsum tau siv cov tshuaj tsis muaj menyuam, thiab cov qauv kev ua haujlwm aseptic yuav tsum ua raws li kom tsis txhob qhia cov kab mob thiab cuam tshuam rau kev loj hlob ntawm tes.