[Daim ntawv theej] Daim duab peb sab Erlenmeyer Flask nrog lub foob lossis lub qhov cua

Cells Loj hlob qeeb hauv Erlenmeyer Flask thiab daws

Dab tsi ua rau qeeb qeeb ntawm cov cell hauv cell shaker flasks
Cells yog rhiab heev rau qhov chaw loj hlob.Thaum culturing hlwb, qee zaum peb ntsib qeeb cell loj hlob.Yog vim li cas?Muaj ntau yam laj thawj rau kev loj hlob qeeb ntawm cov cell hauv cell co flask, feem ntau yog vim li cas hauv qab no:
1. Cov hlwb yuav tsum tau rov kho dua vim qhov hloov pauv ntawm cov kab lis kev cai sib txawv nruab nrab lossis cov ntshav.
2. Cov reagents yog khaws cia tsis raug, thiab qee qhov tsim nyog rau kev loj hlob ntawm tes xws li glutamine lossis cov khoom loj hlob hauv cov kab lis kev cai nruab nrab yog tag nrho lossis tsis muaj lossis raug puas tsuaj.
3. Muaj cov kab mob me me los yog cov kab mob fungal hauv kab lis kev cai hauv cell shaker.
4. Thawj qhov concentration ntawm inoculated hlwb tsawg dhau.
5. Cells muaj hnub nyoog.
6. Mycoplasma paug
Tswv yim daws:
1. Sib piv cov khoom nruab nrab ntawm qhov nruab nrab tshiab thiab qhov qub nruab nrab, thiab sib piv cov ntshav tshiab thiab cov ntshav qabzib qub los txhawb kev loj hlob ntawm tes.Cia cov hlwb maj mam hloov mus rau qhov nruab nrab tshiab.
2. Hloov mus rau hauv cov kab lis kev cai tshiab tshiab, lossis ntxiv cov glutamine thiab kev loj hlob.
3. Muab tshuaj tua kab mob nruab nrab thiab hloov kab lis kev cai yog tias pom muaj kab mob.Cov kab lis kev cai nruab nrab yuav tsum khaws cia ntawm 2-8 ° C hauv qhov tsaus ntuj.Serum-muaj cov nruab nrab tiav yog khaws cia ntawm 2-8 ° C thiab siv li ntawm 2 lub lis piam.
4. Ua kom qhov pib concentration ntawm inoculated hlwb.
5. Hloov cov noob qoob loo tshiab.
6. cais cov kab lis kev cai thiab kuaj pom mycoplasma.Ntxuav lub qhov muag thiab incubator.Yog tias pom muaj kab mob mycoplasma, hloov nrog kab lis kev cai tshiab.